2ZL4

Crystal structure of H.pylori ClpP S99A in complex with the peptide AAAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	91.677, 166.310, 188.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.204
Rwork	0.204
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zl0
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Number of reflections	101077

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	298	32% PEG400, 0.1M HEPES, 0.35M magnesium chloride, pH 7.5, Microbatch, temperature 298K