2ZJW

Crystal structure of human CK2 alpha complexed with Ellagic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-30
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	47.953, 75.883, 78.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.222
Rwork	0.222
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1na7
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Rmerge	0.096
Number of reflections	12518
<I/σ(I)>	29.1
Completeness [%]	100.0
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	25% ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K