2ZIY
Crystal structure of squid rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.319, 108.746, 142.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.200 - 3.700 |
| Rwork | 0.302 |
| R-free | 0.33000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzm |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.064 | 0.775 |
| Number of reflections | 6680 | |
| <I/σ(I)> | 15.1 | 1.3 |
| Completeness [%] | 93.3 | 74.3 |
| Redundancy | 6.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 28% PEG400, 0.1M HEPES, 8% ethylene glycol, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
