2ZIT
Structure of the eEF2-ExoA-NAD+ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 326.920, 69.241, 190.483 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 19.899 - 3.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26600 |
| Starting model (for MR) | eEF2-ExoA(E546H)-NAD+ |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.560 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.164 | 0.583 |
| Number of reflections | 84042 | |
| <I/σ(I)> | 4.6 | 1.4 |
| Completeness [%] | 98.4 | 86.9 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 7% PEG-10000, 3.5mM MPD, 100mM HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
