2ZGW
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A
Replaces: 2E3YExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.308, 82.876, 72.578 |
| Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
| Resolution | 37.250 - 1.500 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.20400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1wqw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.440 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 | 0.274 |
| Number of reflections | 69128 | |
| <I/σ(I)> | 12.8 | 2.1 |
| Completeness [%] | 98.0 | 95.6 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.2 | 295 | PEG20K, Acetate, NaOH, ATP, Biotin, pH 5.2, microbatch, temperature 295K |
