2ZGS
Crystal structure of Agrocybe aegerita lectin AAL mutant L47A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.250, 65.930, 57.950 |
| Unit cell angles | 90.00, 109.09, 90.00 |
Refinement procedure
| Resolution | 24.480 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zgl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.490 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.300 |
| Number of reflections | 29971 | |
| <I/σ(I)> | 19.3 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2M Sodium Chloride, 20% PEG3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
