2ZGR
Crystal structure of AAL mutant L33A in C2 spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.190, 49.270, 50.380 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 24.640 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zgl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.640 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.275 |
| Number of reflections | 13022 | |
| <I/σ(I)> | 21.4 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 2M Ammonium Sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
