2ZGM
Crystal structure of recombinant Agrocybe aegerita lectin,rAAL, complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.090, 58.820, 57.910 |
| Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
| Resolution | 29.460 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zgl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.670 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.119 |
| Number of reflections | 20381 | |
| <I/σ(I)> | 22.6 | 9.2 |
| Completeness [%] | 97.6 | 83.8 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M Ammonium Sulfate, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
