2ZFU

Structure of the methyltransferase-like domain of nucleomethylin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	93
Detector technology	CCD
Collection date	2006-04-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	43.810, 43.810, 403.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.520 - 2.000
R-factor	0.19361
Rwork	0.192
R-free	0.23066
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.237
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.270	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.072	0.284
Number of reflections	28996
Completeness [%]	98.0	92
Redundancy	11	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG 8000, 200mM Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG 8000, 200mM Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG 8000, 200mM Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K