2ZFD
The crystal structure of plant specific calcium binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Detector technology | CCD |
| Collection date | 2007-02-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9796, 0.9798, 0.9645 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.092, 45.481, 92.390 |
| Unit cell angles | 90.00, 92.64, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.18032 |
| Rwork | 0.180 |
| R-free | 0.19162 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.058 | 0.431 |
| Number of reflections | 85565 | |
| Completeness [%] | 96.7 | 91.3 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
