2ZF9
Crystal structure of a type III cohesin module from the cellulosomal ScaE cell-surface anchoring scaffoldin of Ruminococcus flavefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.560, 69.250, 93.030 |
| Unit cell angles | 90.00, 123.37, 90.00 |
Refinement procedure
| Resolution | 23.690 - 1.950 |
| R-factor | 0.16413 |
| Rwork | 0.162 |
| R-free | 0.21122 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.078 | 0.372 |
| Number of reflections | 60499 | |
| <I/σ(I)> | 29.9 | 2.26 |
| Completeness [%] | 98.0 | 89.8 |
| Redundancy | 6.73 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Bis-Tris, 23% PEG 3350, 0.1M calcium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
