2ZEZ
Family 16 Carbohydrate Binding Module-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.425, 99.525, 139.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.23459 |
| Rwork | 0.233 |
| R-free | 0.27203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zew |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 51462 | |
| <I/σ(I)> | 35.3 | 11.6 |
| Completeness [%] | 93.5 | 85.8 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 3.5M sodium formate pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
