2ZEX
Family 16 carbohydrate binding module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.466, 48.829, 49.356 |
| Unit cell angles | 62.03, 84.88, 85.95 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.15679 |
| Rwork | 0.156 |
| R-free | 0.17864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zew |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Number of reflections | 82565 | |
| <I/σ(I)> | 33.3 | 6 |
| Completeness [%] | 94.2 | 87.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 30% PEG3350, 100mM Tris-HCL pH8.3, 200mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
