2ZDO
Crystal structure of IsdG-N7A in complex with hemin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.585, 58.631, 59.692 |
| Unit cell angles | 77.57, 74.39, 75.15 |
Refinement procedure
| Resolution | 56.800 - 1.800 |
| R-factor | 0.19997 |
| Rwork | 0.198 |
| R-free | 0.23446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sqe |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.389 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 49184 | |
| <I/σ(I)> | 6.9 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
