2ZCX

Crystal structure of TetR family transcriptional regulator SCO7815

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2006-10-13
Detector	RIGAKU RAXIS VII
Wavelength(s)	2.29
Spacegroup name	P 41 21 2
Unit cell lengths	72.068, 72.068, 90.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.220
Rwork	0.222
R-free	0.24750
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.170
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.220	2.220
Rmerge	0.060	0.430
Number of reflections	12269
<I/σ(I)>	45.705	8.064
Completeness [%]	99.6	100
Redundancy	27	22.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.8M potassium sodium tartrate, 0.1M Tris-HCl, 1% PEG5000MME, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K