2ZCM
Crystal structure of IcaR, a repressor of the TetR family
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796, 0.9641 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.780, 50.700, 63.340 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.330 |
| Rwork | 0.190 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.054 | 0.289 |
| Number of reflections | 86594 | |
| <I/σ(I)> | 26 | 2.9 |
| Completeness [%] | 98.0 | 89.5 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 12~15% (w/v) PEG 1500, 2.0~2.6M NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
