2ZCI
Structure of a GTP-dependent bacterial PEP-carboxykinase from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.336, 118.055, 152.931 |
| Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
| Resolution | 24.830 - 2.300 |
| R-factor | 0.19226 |
| Rwork | 0.188 |
| R-free | 0.27884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1KHF |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 144542 | |
| <I/σ(I)> | 15 | 3.1 |
| Completeness [%] | 87.1 | 78 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 25% PEG 4000, 0.1M sodium acetate, 0.2M ammonium acetate, 10mM MgCl2, 1mM MnCl2, 2mM EDTA, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
