2ZC0

Crystal structure of an archaeal alanine:glyoxylate aminotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-01
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	77.694, 112.519, 207.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.400 - 2.300
Rwork	0.236
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	79851
<I/σ(I)>	12.4	5.2
Completeness [%]	98.0	87.8
Redundancy	7.1	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Drops (2 micro l) of protein solution (15 mg/ml) containing 5mM L-Ala were mixed with an equal volume of 35-40% 1,4-butanediol, 0.1M zinc acetate, 0.1M imidazol/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	Drops (2 micro l) of protein solution (15 mg/ml) containing 5mM L-Ala were mixed with an equal volume of 35-40% 1,4-butanediol, 0.1M zinc acetate, 0.1M imidazol/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	Drops (2 micro l) of protein solution (15 mg/ml) containing 5mM L-Ala were mixed with an equal volume of 35-40% 1,4-butanediol, 0.1M zinc acetate, 0.1M imidazol/HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K