2ZBS
Crystal structure of human estrogen-related receptor gamma ligand binding domain apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.716, 63.716, 134.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.810 - 1.800 |
| R-factor | 0.20393 |
| Rwork | 0.202 |
| R-free | 0.24443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e2r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.376 |
| Number of reflections | 26307 | |
| <I/σ(I)> | 52 | 4.1 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 8.4 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.4 | VAPOR DIFFUSION, HANGING DROP, temperature 293.4K |
