2ZBM
Crystal Structure of I115M Mutant Cold-Active Protein Tyrosine Phosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2005-05-16 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.172, 77.559, 80.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
R-factor | 0.1216 |
R-free | 0.19730 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.012 |
RMSD bond angle | 0.031 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 54976 | |
Completeness [%] | 97.7 | 84.7 |
Redundancy | 9.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 15% POLYETHYLENEGLYCOL 4000, 0.1M AMMONIUM ACETATE, 0.05M TRIS-HCL (PH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K |