2ZBG
Calcium pump crystal structure with bound AlF4 and TG in the absence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.500, 70.200, 143.400 |
| Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
| Resolution | 11.990 - 2.550 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wpg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.057 | 0.286 |
| Number of reflections | 55231 | |
| <I/σ(I)> | 32.8 | 2 |
| Completeness [%] | 99.8 | 96.3 |
| Redundancy | 7.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |
