2ZAU
Crystal structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.161, 165.198, 167.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.290 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.23900 |
| Structure solution method | MIR |
| RMSD bond length | 0.154 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.097 | 0.429 |
| Number of reflections | 82314 | |
| <I/σ(I)> | 18.3 | 3.66 |
| Completeness [%] | 93.9 | 94.7 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | MPD, AMMONIUM PHOSPHATE, NA-HEPES, pH 7.10, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
