2ZA8
recombinant horse L-chain apoferritin N-terminal deletion mutant (residues 1-8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.210, 180.210, 180.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.400 |
| R-factor | 0.22066 |
| Rwork | 0.220 |
| R-free | 0.23855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aew |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.111 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 52831 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.1M Tris-HCl, 225mM ammonium sulphate, 0.13% cadmium chloride, 25% 2-Metyl-2,4-pentanediol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
