2ZA7
recombinant horse L-chain apoferritin N-terminal deletion mutant (residues 1-4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.280, 180.280, 180.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.520 - 1.400 |
| R-factor | 0.22285 |
| Rwork | 0.222 |
| R-free | 0.24237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aew |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.204 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.520 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 51287 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris-HCl, 0.13% cadmium chloride, 15% PEG4000, 190mM calcium chloride, 5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
