2ZA4
Crystal Structural Analysis of Barnase-barstar Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.388, 110.255, 47.272 |
| Unit cell angles | 90.00, 114.97, 90.00 |
Refinement procedure
| Resolution | 43.360 - 1.580 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.047 | 0.246 |
| Number of reflections | 61328 | |
| <I/σ(I)> | 35.1 | 5 |
| Completeness [%] | 99.3 | 90.9 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
