2Z9W
Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-11 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.732, 68.732, 311.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.840 - 1.700 |
| R-factor | 0.15543 |
| Rwork | 0.154 |
| R-free | 0.18051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2Z9U |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.084 | 0.386 |
| Number of reflections | 100019 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.05 | 277 | 0.1M HEPES, 2M Ammonium sulfate, 10mM Pyridoxal, pH 8.05, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
