2Z8Z
Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-19 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.071784 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.879, 84.404, 87.012 |
| Unit cell angles | 90.00, 96.50, 90.00 |
Refinement procedure
| Resolution | 32.120 - 1.800 |
| R-factor | 0.17512 |
| Rwork | 0.174 |
| R-free | 0.20493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8x |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.771 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.074 |
| Number of reflections | 63435 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 20000, 0.1M MES, 0.2M calcium acetate, 5mM zinc acetate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
