2Z8W
Structure of an IgNAR-AMA1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96426 |
| Spacegroup name | P 31 |
| Unit cell lengths | 76.480, 76.480, 140.978 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.330 - 2.450 |
| R-factor | 0.19822 |
| Rwork | 0.189 |
| R-free | 0.28183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1Z40 and 1VER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.230 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.130 | 0.570 |
| Number of reflections | 28622 | |
| <I/σ(I)> | 14.4 | 1.6 |
| Redundancy | 5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.1M phosphate citrate pH 4.2, 0.2M NaCl, 20% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
