2Z8U
Methanococcus jannaschii TBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.242, 55.532, 123.434 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.22734 |
| Rwork | 0.226 |
| R-free | 0.25911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mp9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.290 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.350 |
| Number of reflections | 57094 | |
| <I/σ(I)> | 9.2 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG MME 2000, sodium acetate, ammonium sulfate, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
