2Z8S
Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 2007-01-21 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.617, 105.152, 100.668 |
| Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
| Resolution | 26.150 - 2.500 |
| R-factor | 0.16568 |
| Rwork | 0.162 |
| R-free | 0.24169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.259 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.200 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 37253 | |
| <I/σ(I)> | 12.4 | 2.41 |
| Completeness [%] | 93.3 | 70 |
| Redundancy | 1.53 | 1.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 55% MPD, 0.1M TRIS, pH8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
