2Z7C
Crystal structure of chromatin protein alba from hyperthermophilic archaeon pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.262, 51.974, 173.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.550 - 2.800 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.31800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y9x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.790 | 2.800 |
Number of reflections | 8238 | |
Completeness [%] | 83.1 | 51 |
Redundancy | 5.1 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 15% PEGMME 550, 0.2M Sodium chloride, 0.1M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |