2Z70
E.coli RNase 1 in complex with d(CGCGATCGCG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8125 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.556, 49.758, 143.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.19363 |
| Rwork | 0.190 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pqx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.357 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 | 0.319 |
| Number of reflections | 28734 | |
| <I/σ(I)> | 16.9 | 4.2 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 20% PEG8000, 0.1M Sodium acetate, 1mM MgCl2, 0.1M MES, pH6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG8000 | ||
| 10 | 1 | 2 | HOH | ||
| 2 | 1 | 1 | Sodium acetate | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | PEG8000 | ||
| 7 | 1 | 2 | Sodium acetate | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | MES |
