2Z6Y
Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.889, 95.829, 74.011 |
| Unit cell angles | 90.00, 115.62, 90.00 |
Refinement procedure
| Resolution | 19.510 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z6z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.118 | 0.371 |
| Number of reflections | 53536 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.3 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 20% PEG 3000, 0.2M Ca(acetate)2, 0.1M Tris/HCl (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 291K |
