2Z68
Crystal Structure Of An Artificial Metalloprotein: Cr[N-salicylidene-4-amino-3-hydroxyhydrocinnamic acid]/Wild Type Heme oxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2005-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.656, 63.057, 77.964 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 29.770 - 1.580 |
| R-factor | 0.18049 |
| Rwork | 0.178 |
| R-free | 0.22493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wzd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.061 | |
| Number of reflections | 53304 | |
| <I/σ(I)> | 23.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES pH6.5, 0.2mM Ammonium sulfate, 30% w/w PEG 2000 methyl mono ester, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
