2Z67

Crystal structure of archaeal O-phosphoseryl-tRNA(Sec) selenium transferase (SepSecS)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791, 0.9793, 0.9820, 0.9770
Spacegroup name	P 1 21 1
Unit cell lengths	75.745, 108.143, 110.390
Unit cell angles	90.00, 96.50, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.208
Rwork	0.208
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.500	2.500
Number of reflections	59938
<I/σ(I)>	17	2.9
Completeness [%]	97.9	86.1
Redundancy	5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K