2Z5W
tBclA, a recombinant spore surface protein from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 138 |
| Detector technology | CCD |
| Collection date | 2006-06-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96426 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 68.144, 68.144, 163.178 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.820 - 1.350 |
| R-factor | 0.17485 |
| Rwork | 0.174 |
| R-free | 0.19107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1WCK |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.407 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 1.380 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.035 | 0.850 |
| Number of reflections | 43616 | |
| <I/σ(I)> | 74.9 | 1.1 |
| Redundancy | 33 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES (pH 6.5), 17.5% EtOH, 5% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
