2Z5N
Complex of Transportin 1 with hnRNP D NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.683, 118.954, 151.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.220 - 3.200 |
| R-factor | 0.23493 |
| Rwork | 0.232 |
| R-free | 0.29525 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z5o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.082 | 0.541 |
| Number of reflections | 18626 | |
| <I/σ(I)> | 10.2 | 1.7 |
| Completeness [%] | 91.9 | 56.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1M Tris-HCl pH 8.8, 16-20%(w/v) PEG 8000, 0.1M KCl, 0.1M NaH2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
