2Z5H
Crystal structure of the head-to-tail junction of tropomyosin complexed with a fragment of TnT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-02-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 86.809, 158.308, 163.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.960 - 2.890 |
| R-factor | 0.23729 |
| Rwork | 0.237 |
| R-free | 0.24701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.124 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.960 | 3.000 |
| High resolution limit [Å] | 2.890 | 2.900 |
| Rmerge | 0.077 | 0.317 |
| Number of reflections | 25479 | |
| <I/σ(I)> | 9.6 | 2.8 |
| Completeness [%] | 96.5 | 95.5 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
