2Z51
Crystal structure of Arabidopsis CnfU involved in iron-sulfur cluster biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-17 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 43.546, 57.394, 61.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.310 - 1.350 |
| R-factor | 0.13671 |
| Rwork | 0.135 |
| R-free | 0.17338 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.030 |
| RMSD bond angle | 1.634 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.310 | 1.383 |
| High resolution limit [Å] | 1.350 | 1.348 |
| Number of reflections | 34580 | |
| Completeness [%] | 99.9 | 97.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 1000, MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
