2Z4U

Crystal structure of wild type PD-L4 from Phytolacca dioica leaves

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	43.521, 58.813, 98.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.100
Rwork	0.122
R-free	0.14600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qcg
RMSD bond length	0.017
RMSD bond angle	0.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	Il (Milione)
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.200
High resolution limit [Å]	1.100	1.100
Number of reflections	102812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	20% PEG4000, 10% isopropanol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, temperature 298K