2Z4Q

Crystal structure of a murine antibody FAB 528

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	95
Detector technology	CCD
Collection date	2005-11-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 62
Unit cell lengths	126.600, 126.600, 68.278
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.950 - 2.300
R-factor	0.191
Rwork	0.191
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wt5 1e4x
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.062	0.273
Number of reflections	27846
Completeness [%]	99.8
Redundancy	10.7	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	1.4M ammonium sulfate, 0.01M cadmium chloride, 0.1M Tris-HCl, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K