2Z44

Crystal Structure of Selenomethionine-labeled ORF134

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2005-06-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97923, 0.97946, 0.96423
Spacegroup name	P 31 1 2
Unit cell lengths	56.461, 56.461, 85.167
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.800 - 2.500
R-factor	0.20823
Rwork	0.205
R-free	0.28050
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.463
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0034)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.109	0.461
Number of reflections	5247
Completeness [%]	98.7	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50% PEG 200, 0.1M phosphate-citrate pH 4.2, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K