2Z3X
Structure of a Protein-DNA Complex Essential for DNA Protection in Spore of Bacillus Species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.962, 86.962, 144.669 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.930 - 2.100 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.292 |
| Number of reflections | 17711 | |
| <I/σ(I)> | 15.9 | 2.7 |
| Completeness [%] | 90.5 | 59.4 |
| Redundancy | 7.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 0.01M magnesium sulfate, 0.05M sodium cacodylate, 1.4M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 0.01M magnesium sulfate, 0.05M sodium cacodylate, 1.4M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | magnesium sulfate | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | ammonium sulfate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | magnesium sulfate | ||
| 6 | 1 | 2 | odium cacodylate | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | H2O |
