2Z3B
Crystal Structure of Bacillus Subtilis CodW, a non-canonical HslV-like peptidase with an impaired catalytic apparatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-27 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.12714 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.050, 106.800, 152.700 |
| Unit cell angles | 90.00, 112.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.528 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 90904 | |
| <I/σ(I)> | 17.2 | 2.76 |
| Completeness [%] | 95.3 | 88.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | NA HEPES, NA CITRATE, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
