2Z3A
Crystal Structure of Bacillus Subtilis CodW, a non-canonical HslV-like peptidase with an impaired catalytic apparatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-26 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.127 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.130, 164.420, 95.900 |
Unit cell angles | 90.00, 111.53, 90.00 |
Refinement procedure
Resolution | 40.370 - 3.000 |
R-factor | 0.247 |
Rwork | 0.245 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.117 | |
Number of reflections | 44150 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 98.1 | 97.6 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | MPD, SODIUM CITRATE, GLYCEROL, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |