2Z2R

Nucleosome assembly proteins I (NAP-1, 74-365)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.1
Spacegroup name	P 42 21 2
Unit cell lengths	125.000, 125.000, 146.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.200
Rwork	0.260
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ayu
RMSD bond length	0.009
RMSD bond angle	1.537
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200	3.200
Rmerge	0.070	0.380
Number of reflections	17067
<I/σ(I)>	14	3.5
Completeness [%]	86.4	92
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	40% MPD, 0.1M sodium citrate, 0.2M ammonium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K