2Z1B
Crystal Structure of 5-aminolevulinic acid dehydratase (ALAD) from Mus musculs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97955 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 240.920, 240.920, 103.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.400 - 3.300 |
| R-factor | 0.278 |
| Rwork | 0.278 |
| R-free | 0.35300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z0i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 27448 | |
| <I/σ(I)> | 19.3 | 4.01 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5M Li2SO4, 0.1M HEPES-Na (pH7.5), 9.5% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
