2Z18
Phase transition of monoclinic lysozyme crystal soaked in a 10% NaCl solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-12-11 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.622, 60.588, 31.223 |
| Unit cell angles | 90.00, 111.44, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.150 |
| Rwork | 0.156 |
| R-free | 0.17600 |
| Structure solution method | SHELX-97 |
| Starting model (for MR) | 2d4j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data reduction software | SAINT (Plus) |
| Data scaling software | SAINT (Plus) |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.104 | 0.090 |
| Number of reflections | 32729 | |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.73 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Dehydration induced phase transition | 293 | 10% NaCl, temperature-regulated nitrogen gas, Dehydration induced phase transition, temperature 293K |
