2Z0I
Crystal Structure of 5-aminolevulinic acid dehydratase (ALAD) from Mus musculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 121.510, 121.510, 196.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.680 - 3.200 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e51 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 12792 | |
| <I/σ(I)> | 14.2 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | TRIS, PEG 400, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
