2Z0F
Crystal structure of putative phosphoglucomutase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-23 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9793, 1.0000, 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.676, 102.297, 87.462 |
| Unit cell angles | 90.00, 117.19, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.520 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 41420 | |
| <I/σ(I)> | 20 | 3.3 |
| Completeness [%] | 97.8 | 86.2 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 293 | 12% PEG6000, 0.2M Lithium Sulfate, 0.1M Na3 Citrate, pH 5.6, temperature 293K |
